P 125 Lack of cytotoxic effect of ganciclovir and acyclovir on rabbit corneal epithelial cells in vitro

EVALUATION OF TOPICAL KETOCONAZOL $ EFFECTS ON THE REGENERATION OF COP.NRAL EPITHELIUM EXPERIMENTAL TUDY IN RABBITS. JORGE E.N.l JORGE E.C.1 MOUES SILVA M.R.B.1 VANE, L.A.2 1 Dep,mlenL Of GQhth.hology, ""iver‘iddc B*t.d"a, Paulill* (S.PS"lO, Brartt) 2 Departme"t or A"elttwlolo,y, "niversldade Eer.d"at Fxu,ista (S.PS"lO, Br*r*l) e 29 male rabbits were submitted to bilateral epithelisl sblstio" of corness induced by mechanical and chemical meChods.Tbc toxicity of fnpicsl Ketoccnszole (KCZ). used at 2 different concenrrstions. 2% and 4X, wss evalusted, considering rhe following parameters: rate of re-epithelizstion and histopsthologicsl chsnges of rhe carries. We tested rhe applicability in this experimental mode of attributes like rhe chsrseceristics of the cicaCrization process. the shape of the cicstrizsfion process and rhe rate between Che minimum and maximum dismefer of the re-epithelisseior ores. m The snimsls were divided in 4 groups: GI: received 2% KC2 in the right eye (GID); GII: 4% KC2 in the right eye (G2D); GIII: control colyrium in the right eye (630). GIV: no drops were instilled in the right eye. The left eyes of all animals used in th6 experimencsl gro;ps listed above did not receive any kind of rrej tmenr, after rhe epithelisl ablation. They were considered the biological co"rrol.of the opposite crested eye. w They were studied by qusnfitsrive and quslitsrive methods with atstis~icsl snslysia of sues under the cicstrizstio” line, the rate between the minimum and maximum diameter of ehe re-epirhelizstion ares and irs shape. All the cor"ess had hisfopsthol~ gicsl analysis. Conclusions The results demostrsted that the use of 2% Ketoconszolr has no toxic effect to the cicsrrizstion process of Che cornea, when compsred tn the control groups. In ccntrsst. the use of 4% Ketnconszole caused toxic effects compared eo the control groups, considering the parameters evaluated in this experimental study. Shape had greater sensibility to show differences among different groups rhsn rate between dismetere. 4% Kerccons~ole had toxic effects et hlstopsrholo~ical analysis.